Cellular cloning

Clone a cell consists of a group of them from a single. In the case of unicellular bacteria and yeast, this process is very simple and only requires the inoculation of suitable products. However, in the case of cultured cells in multicellular organisms, the cloning of cells is a difficult task, since these cells need the medium very specific conditions. A useful tissue culture technique used to clone distinct lineages of cells is the use of cloning (cylinders) rings. According to this technique, a grouping of unicellular cells that have been exposed to a mutagenic agent or a medicine used for the selection are put in a high dilution to create isolated colonies; each coming from a single potentially and clonally differentiated cell. In a first stage of growth, when the colonies have only a few cells; submerge sterile polystyrene fat rings, and put on a colony of single along with a small amount of trypsin. Cells that are cloned, are collected within the ring and is often a new container continue their growth in a natural way.